YPL022W / RAD1

Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL022W / RAD1

Click on Significant Values for Screen Details ID:SGTC_39|Compound:0986-0283|FD-Score:-5.68|P-value:4.50E-9 ID:SGTC_40|Compound:0327-0103|FD-Score:4.53|P-value:2.46E-6 ID:SGTC_41|Compound:0478-0632|FD-Score:-4.95|P-value:2.78E-7 ID:SGTC_44|Compound:0929-0055|FD-Score:8.64|P-value:1.39E-18 ID:SGTC_45|Compound:0kpi-0036|FD-Score:-4.02|P-value:2.35E-5 ID:SGTC_130|Compound:0136-0219|FD-Score:3.11|P-value:8.51E-4 ID:SGTC_153|Compound:4077-0274|FD-Score:3.60|P-value:1.39E-4 ID:SGTC_247|Compound:mechlorethamine|FD-Score:7.16|P-value:2.39E-13 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:27.10|P-value:3.25E-165 ID:SGTC_529|Compound:4076-0302|FD-Score:-3.07|P-value:9.48E-4 ID:SGTC_541|Compound:4513-1321|FD-Score:3.09|P-value:9.29E-4 ID:SGTC_867|Compound:0518-0118|FD-Score:3.19|P-value:6.51E-4 ID:SGTC_960|Compound:1222-0186|FD-Score:-3.17|P-value:6.64E-4 ID:SGTC_1053|Compound:3366-3204|FD-Score:-3.12|P-value:7.83E-4 ID:SGTC_1070|Compound:indatraline|FD-Score:3.72|P-value:8.81E-5 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:-3.68|P-value:9.65E-5 ID:SGTC_1147|Compound:3809-1045|FD-Score:3.15|P-value:7.48E-4 ID:SGTC_1149|Compound:3909-7961|FD-Score:3.57|P-value:1.56E-4 ID:SGTC_1155|Compound:4088-0394|FD-Score:3.71|P-value:8.99E-5 ID:SGTC_1180|Compound:1193-0155|FD-Score:4.66|P-value:1.28E-6 ID:SGTC_1195|Compound:1502-0147|FD-Score:3.93|P-value:3.70E-5 ID:SGTC_1198|Compound:1612-1005|FD-Score:4.13|P-value:1.59E-5 ID:SGTC_1224|Compound:0823-0416|FD-Score:-3.20|P-value:5.95E-4 ID:SGTC_1352|Compound:1496-0991|FD-Score:4.70|P-value:1.04E-6 ID:SGTC_1425|Compound:4048-4433|FD-Score:3.17|P-value:6.94E-4 ID:SGTC_1908|Compound:st060353|FD-Score:4.90|P-value:3.83E-7 ID:SGTC_1919|Compound:st067113|FD-Score:6.65|P-value:9.53E-12 ID:SGTC_2088|Compound:5278280|FD-Score:3.39|P-value:3.18E-4 ID:SGTC_2200|Compound:6600604|FD-Score:3.21|P-value:6.10E-4 ID:SGTC_2238|Compound:6671620|FD-Score:-3.86|P-value:4.66E-5 ID:SGTC_2317|Compound:5986382|FD-Score:4.33|P-value:6.31E-6 ID:SGTC_2328|Compound:6201408|FD-Score:3.48|P-value:2.22E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:4.20|P-value:1.12E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.15|P-value:7.51E-4 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:-3.06|P-value:9.79E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.70|P-value:9.60E-5 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:-3.94|P-value:3.28E-5 ID:SGTC_2576|Compound:guaiol|FD-Score:-3.80|P-value:5.93E-5 ID:SGTC_2585|Compound:alliin|FD-Score:7.23|P-value:1.47E-13 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:5.58|P-value:9.21E-9 ID:SGTC_2612|Compound:dictamnine|FD-Score:-3.18|P-value:6.46E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:-4.42|P-value:3.91E-6 ID:SGTC_2677|Compound:mitomycin C|FD-Score:5.32|P-value:3.89E-8 ID:SGTC_2690|Compound:usnic acid|FD-Score:5.61|P-value:7.64E-9 ID:SGTC_2692|Compound:st077426|FD-Score:-3.06|P-value:9.64E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:3.69|P-value:1.01E-4 ID:SGTC_2842|Compound:9000675|FD-Score:3.27|P-value:4.96E-4 ID:SGTC_2972|Compound:9093619|FD-Score:4.21|P-value:1.06E-5 ID:SGTC_2987|Compound:9018338|FD-Score:3.12|P-value:8.40E-4 ID:SGTC_3137|Compound:9097352|FD-Score:-3.28|P-value:4.44E-4 ID:SGTC_39|Compound:0986-0283|FD-Score:-5.68|P-value:4.50E-9 ID:SGTC_40|Compound:0327-0103|FD-Score:4.53|P-value:2.46E-6 ID:SGTC_41|Compound:0478-0632|FD-Score:-4.95|P-value:2.78E-7 ID:SGTC_44|Compound:0929-0055|FD-Score:8.64|P-value:1.39E-18 ID:SGTC_45|Compound:0kpi-0036|FD-Score:-4.02|P-value:2.35E-5 ID:SGTC_130|Compound:0136-0219|FD-Score:3.11|P-value:8.51E-4 ID:SGTC_153|Compound:4077-0274|FD-Score:3.60|P-value:1.39E-4 ID:SGTC_247|Compound:mechlorethamine|FD-Score:7.16|P-value:2.39E-13 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:27.10|P-value:3.25E-165 ID:SGTC_529|Compound:4076-0302|FD-Score:-3.07|P-value:9.48E-4 ID:SGTC_541|Compound:4513-1321|FD-Score:3.09|P-value:9.29E-4 ID:SGTC_867|Compound:0518-0118|FD-Score:3.19|P-value:6.51E-4 ID:SGTC_960|Compound:1222-0186|FD-Score:-3.17|P-value:6.64E-4 ID:SGTC_1053|Compound:3366-3204|FD-Score:-3.12|P-value:7.83E-4 ID:SGTC_1070|Compound:indatraline|FD-Score:3.72|P-value:8.81E-5 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:-3.68|P-value:9.65E-5 ID:SGTC_1147|Compound:3809-1045|FD-Score:3.15|P-value:7.48E-4 ID:SGTC_1149|Compound:3909-7961|FD-Score:3.57|P-value:1.56E-4 ID:SGTC_1155|Compound:4088-0394|FD-Score:3.71|P-value:8.99E-5 ID:SGTC_1180|Compound:1193-0155|FD-Score:4.66|P-value:1.28E-6 ID:SGTC_1195|Compound:1502-0147|FD-Score:3.93|P-value:3.70E-5 ID:SGTC_1198|Compound:1612-1005|FD-Score:4.13|P-value:1.59E-5 ID:SGTC_1224|Compound:0823-0416|FD-Score:-3.20|P-value:5.95E-4 ID:SGTC_1352|Compound:1496-0991|FD-Score:4.70|P-value:1.04E-6 ID:SGTC_1425|Compound:4048-4433|FD-Score:3.17|P-value:6.94E-4 ID:SGTC_1908|Compound:st060353|FD-Score:4.90|P-value:3.83E-7 ID:SGTC_1919|Compound:st067113|FD-Score:6.65|P-value:9.53E-12 ID:SGTC_2088|Compound:5278280|FD-Score:3.39|P-value:3.18E-4 ID:SGTC_2200|Compound:6600604|FD-Score:3.21|P-value:6.10E-4 ID:SGTC_2238|Compound:6671620|FD-Score:-3.86|P-value:4.66E-5 ID:SGTC_2317|Compound:5986382|FD-Score:4.33|P-value:6.31E-6 ID:SGTC_2328|Compound:6201408|FD-Score:3.48|P-value:2.22E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:4.20|P-value:1.12E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.15|P-value:7.51E-4 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:-3.06|P-value:9.79E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.70|P-value:9.60E-5 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:-3.94|P-value:3.28E-5 ID:SGTC_2576|Compound:guaiol|FD-Score:-3.80|P-value:5.93E-5 ID:SGTC_2585|Compound:alliin|FD-Score:7.23|P-value:1.47E-13 ID:SGTC_2590|Compound:3,4',5',6',7'-pentamethoxyflavone|FD-Score:5.58|P-value:9.21E-9 ID:SGTC_2612|Compound:dictamnine|FD-Score:-3.18|P-value:6.46E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:-4.42|P-value:3.91E-6 ID:SGTC_2677|Compound:mitomycin C|FD-Score:5.32|P-value:3.89E-8 ID:SGTC_2690|Compound:usnic acid|FD-Score:5.61|P-value:7.64E-9 ID:SGTC_2692|Compound:st077426|FD-Score:-3.06|P-value:9.64E-4 ID:SGTC_2779|Compound:methotrexate|FD-Score:3.69|P-value:1.01E-4 ID:SGTC_2842|Compound:9000675|FD-Score:3.27|P-value:4.96E-4 ID:SGTC_2972|Compound:9093619|FD-Score:4.21|P-value:1.06E-5 ID:SGTC_2987|Compound:9018338|FD-Score:3.12|P-value:8.40E-4 ID:SGTC_3137|Compound:9097352|FD-Score:-3.28|P-value:4.44E-4

Top fitness defect scores for YPL022W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_267 4-Nitroquinoline-1-oxide (4-NQO) 27.10 44.4 nM 3.25E-165 DNA damage response 3 5.90E-162
2 SGTC_44 0929-0055 8.64 61.2 µM 1.39E-18 2 2.87E-18
3 SGTC_2585 alliin 7.23 100.0 µM 1.47E-13 3 2.44E-13
4 SGTC_247 mechlorethamine 7.16 29.7 µM 2.39E-13 DNA damage response 2 3.91E-13
5 SGTC_1919 st067113 6.65 52.3 µM 9.53E-12 TSC3-RPN4 5 1.46E-11
6 SGTC_2690 usnic acid 5.61 100.0 µM 7.64E-9 6 1.03E-8
7 SGTC_2590 3,4',5',6',7'-pentamethoxyflavone 5.58 100.0 µM 9.21E-9 60S ribosome export 4 1.24E-8
8 SGTC_2677 mitomycin C 5.32 100.0 µM 3.89E-8 DNA damage response 6 5.08E-8
9 SGTC_1908 st060353 4.90 56.5 µM 3.83E-7 RPP1 & pyrimidine depletion 22 4.80E-7
10 SGTC_1352 1496-0991 4.70 99.0 µM 1.04E-6 tubulin folding & SWR complex 15 1.28E-6
11 SGTC_1180 1193-0155 4.66 285.0 µM 1.28E-6 5 1.57E-6
12 SGTC_40 0327-0103 4.53 19.8 µM 2.46E-6 16 2.98E-6
13 SGTC_2317 5986382 4.33 200.0 µM 6.31E-6 9 7.49E-6
14 SGTC_2972 9093619 4.21 59.9 µM 1.06E-5 16 1.25E-5
15 SGTC_2405 ticlatone 4.20 3.5 µM 1.12E-5 62 1.31E-5
16 SGTC_1198 1612-1005 4.13 86.7 µM 1.59E-5 8 1.85E-5
17 SGTC_1195 1502-0147 3.93 41.1 µM 3.70E-5 29 4.25E-5
18 SGTC_1070 indatraline 3.72 8.8 µM 8.81E-5 60S ribosome export 31 9.98E-5
19 SGTC_1155 4088-0394 3.71 48.2 µM 8.99E-5 38 1.02E-4
20 SGTC_2566 crystal violet carbinol 3.70 226.0 nM 9.60E-5 47 1.09E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.626 0 YML095C RAD10 hom Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein
0.610 0 YGR258C RAD2 hom Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein
0.601 0 YMR201C RAD14 hom Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein
0.466 8.85E-181 YER162C RAD4 hom Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins
0.365 3.24E-106 YDR092W UBC13 hom Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus
0.339 3.85E-91 YEL037C RAD23 hom Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover
0.285 8.63E-64 YGL087C MMS2 hom Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress
0.237 3.75E-44 YLR032W RAD5 hom DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
0.210 1.10E-34 YCR066W RAD18 hom E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA
0.200 1.39E-31 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.190 1.35E-28 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.162 5.11E-21 YIR002C MPH1 hom 3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases
0.161 7.74E-21 YPL020C ULP1 het Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; sequestered to the nucleolus under stress conditions
0.151 1.17E-18 YJL092W SRS2 hom DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability
0.151 1.71E-18 YBR099C_d YBR099C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4