YPL208W / RKM1

SET-domain lysine-N-methyltransferase, catalyzes the formation of dimethyllysine residues on the large ribsomal subunit proteins L23a (Rpl23Ap and Rpl23Bp) and L18 (Rps18Ap and Rps18Bp)

Zygosity: Homozygous strain
fixedexpanded
Profile for YPL208W / RKM1

Click on Significant Values for Screen Details ID:SGTC_33|Compound:0986-0033|FD-Score:-3.14|P-value:6.86E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.57|P-value:1.59E-6 ID:SGTC_66|Compound:0109-0187|FD-Score:3.64|P-value:1.06E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:3.31|P-value:3.87E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.16|P-value:6.57E-4 ID:SGTC_175|Compound:3793-3120|FD-Score:3.36|P-value:3.16E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-5.10|P-value:1.00E-7 ID:SGTC_312|Compound:4092-0653|FD-Score:-4.23|P-value:7.99E-6 ID:SGTC_344|Compound:k064-0035|FD-Score:3.12|P-value:7.65E-4 ID:SGTC_518|Compound:1021-0119|FD-Score:3.31|P-value:3.85E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:3.37|P-value:3.10E-4 ID:SGTC_705|Compound:1124-0212|FD-Score:-3.28|P-value:4.17E-4 ID:SGTC_777|Compound:1315-0167|FD-Score:-5.24|P-value:4.68E-8 ID:SGTC_786|Compound:4544-0069|FD-Score:4.97|P-value:2.22E-7 ID:SGTC_787|Compound:4152-0075|FD-Score:-3.73|P-value:6.97E-5 ID:SGTC_791|Compound:1348-1292|FD-Score:5.03|P-value:1.56E-7 ID:SGTC_792|Compound:4534-1957|FD-Score:-3.50|P-value:1.80E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:6.36|P-value:5.12E-11 ID:SGTC_864|Compound:0469-0573|FD-Score:3.98|P-value:2.61E-5 ID:SGTC_882|Compound:0843-0131|FD-Score:4.04|P-value:2.03E-5 ID:SGTC_969|Compound:1313-0238|FD-Score:3.42|P-value:2.60E-4 ID:SGTC_978|Compound:1416-0512|FD-Score:4.83|P-value:4.45E-7 ID:SGTC_1192|Compound:1488-0095|FD-Score:3.63|P-value:1.11E-4 ID:SGTC_1284|Compound:0929-0043|FD-Score:3.35|P-value:3.36E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-5.74|P-value:2.38E-9 ID:SGTC_1677|Compound:st019204|FD-Score:3.72|P-value:7.96E-5 ID:SGTC_1679|Compound:4130-1276|FD-Score:-3.46|P-value:2.09E-4 ID:SGTC_1728|Compound:st035614|FD-Score:5.95|P-value:7.35E-10 ID:SGTC_1730|Compound:st033808|FD-Score:3.11|P-value:7.81E-4 ID:SGTC_1745|Compound:st081495|FD-Score:-3.86|P-value:4.05E-5 ID:SGTC_1754|Compound:st042065|FD-Score:-3.61|P-value:1.16E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-3.27|P-value:4.20E-4 ID:SGTC_2003|Compound:4023581|FD-Score:8.08|P-value:9.95E-17 ID:SGTC_2200|Compound:6600604|FD-Score:3.30|P-value:3.94E-4 ID:SGTC_2432|Compound:5630864|FD-Score:3.82|P-value:5.14E-5 ID:SGTC_2434|Compound:5670830|FD-Score:4.69|P-value:9.20E-7 ID:SGTC_2461|Compound:5755302|FD-Score:3.31|P-value:3.86E-4 ID:SGTC_2481|Compound:5478948|FD-Score:3.70|P-value:8.66E-5 ID:SGTC_2485|Compound:5633444|FD-Score:-3.32|P-value:3.54E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.36|P-value:3.03E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:-3.82|P-value:4.85E-5 ID:SGTC_2689|Compound:tacrolimus|FD-Score:3.26|P-value:4.62E-4 ID:SGTC_2740|Compound:felodipine|FD-Score:3.09|P-value:8.62E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:-3.25|P-value:4.52E-4 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:4.51|P-value:2.26E-6 ID:SGTC_2775|Compound:cyproheptadine|FD-Score:4.18|P-value:1.07E-5 ID:SGTC_2942|Compound:9052777|FD-Score:4.31|P-value:5.94E-6 ID:SGTC_2945|Compound:9067681|FD-Score:6.33|P-value:5.92E-11 ID:SGTC_3037|Compound:9091026|FD-Score:-3.93|P-value:3.11E-5 ID:SGTC_3211|Compound:9128781|FD-Score:4.08|P-value:1.69E-5 ID:SGTC_3224|Compound:9130643|FD-Score:3.21|P-value:5.54E-4 ID:SGTC_3272|Compound:9137413|FD-Score:3.51|P-value:1.81E-4 ID:SGTC_33|Compound:0986-0033|FD-Score:-3.14|P-value:6.86E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-4.57|P-value:1.59E-6 ID:SGTC_66|Compound:0109-0187|FD-Score:3.64|P-value:1.06E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:3.31|P-value:3.87E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.16|P-value:6.57E-4 ID:SGTC_175|Compound:3793-3120|FD-Score:3.36|P-value:3.16E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-5.10|P-value:1.00E-7 ID:SGTC_312|Compound:4092-0653|FD-Score:-4.23|P-value:7.99E-6 ID:SGTC_344|Compound:k064-0035|FD-Score:3.12|P-value:7.65E-4 ID:SGTC_518|Compound:1021-0119|FD-Score:3.31|P-value:3.85E-4 ID:SGTC_625|Compound:k213-0058|FD-Score:3.37|P-value:3.10E-4 ID:SGTC_705|Compound:1124-0212|FD-Score:-3.28|P-value:4.17E-4 ID:SGTC_777|Compound:1315-0167|FD-Score:-5.24|P-value:4.68E-8 ID:SGTC_786|Compound:4544-0069|FD-Score:4.97|P-value:2.22E-7 ID:SGTC_787|Compound:4152-0075|FD-Score:-3.73|P-value:6.97E-5 ID:SGTC_791|Compound:1348-1292|FD-Score:5.03|P-value:1.56E-7 ID:SGTC_792|Compound:4534-1957|FD-Score:-3.50|P-value:1.80E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:6.36|P-value:5.12E-11 ID:SGTC_864|Compound:0469-0573|FD-Score:3.98|P-value:2.61E-5 ID:SGTC_882|Compound:0843-0131|FD-Score:4.04|P-value:2.03E-5 ID:SGTC_969|Compound:1313-0238|FD-Score:3.42|P-value:2.60E-4 ID:SGTC_978|Compound:1416-0512|FD-Score:4.83|P-value:4.45E-7 ID:SGTC_1192|Compound:1488-0095|FD-Score:3.63|P-value:1.11E-4 ID:SGTC_1284|Compound:0929-0043|FD-Score:3.35|P-value:3.36E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-5.74|P-value:2.38E-9 ID:SGTC_1677|Compound:st019204|FD-Score:3.72|P-value:7.96E-5 ID:SGTC_1679|Compound:4130-1276|FD-Score:-3.46|P-value:2.09E-4 ID:SGTC_1728|Compound:st035614|FD-Score:5.95|P-value:7.35E-10 ID:SGTC_1730|Compound:st033808|FD-Score:3.11|P-value:7.81E-4 ID:SGTC_1745|Compound:st081495|FD-Score:-3.86|P-value:4.05E-5 ID:SGTC_1754|Compound:st042065|FD-Score:-3.61|P-value:1.16E-4 ID:SGTC_1818|Compound:st052136|FD-Score:-3.27|P-value:4.20E-4 ID:SGTC_2003|Compound:4023581|FD-Score:8.08|P-value:9.95E-17 ID:SGTC_2200|Compound:6600604|FD-Score:3.30|P-value:3.94E-4 ID:SGTC_2432|Compound:5630864|FD-Score:3.82|P-value:5.14E-5 ID:SGTC_2434|Compound:5670830|FD-Score:4.69|P-value:9.20E-7 ID:SGTC_2461|Compound:5755302|FD-Score:3.31|P-value:3.86E-4 ID:SGTC_2481|Compound:5478948|FD-Score:3.70|P-value:8.66E-5 ID:SGTC_2485|Compound:5633444|FD-Score:-3.32|P-value:3.54E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.36|P-value:3.03E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:-3.82|P-value:4.85E-5 ID:SGTC_2689|Compound:tacrolimus|FD-Score:3.26|P-value:4.62E-4 ID:SGTC_2740|Compound:felodipine|FD-Score:3.09|P-value:8.62E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:-3.25|P-value:4.52E-4 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:4.51|P-value:2.26E-6 ID:SGTC_2775|Compound:cyproheptadine|FD-Score:4.18|P-value:1.07E-5 ID:SGTC_2942|Compound:9052777|FD-Score:4.31|P-value:5.94E-6 ID:SGTC_2945|Compound:9067681|FD-Score:6.33|P-value:5.92E-11 ID:SGTC_3037|Compound:9091026|FD-Score:-3.93|P-value:3.11E-5 ID:SGTC_3211|Compound:9128781|FD-Score:4.08|P-value:1.69E-5 ID:SGTC_3224|Compound:9130643|FD-Score:3.21|P-value:5.54E-4 ID:SGTC_3272|Compound:9137413|FD-Score:3.51|P-value:1.81E-4

Top fitness defect scores for YPL208W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2003 4023581 8.08 200.0 µM 9.95E-17 cell wall signaling 7 3.15E-16
2 SGTC_800 3448-7362 6.36 155.0 µM 5.12E-11 translation 36 1.04E-10
3 SGTC_2945 9067681 6.33 61.8 µM 5.92E-11 3 1.20E-10
4 SGTC_1728 st035614 5.95 33.8 µM 7.35E-10 8 1.37E-9
5 SGTC_791 1348-1292 5.03 225.0 µM 1.56E-7 17 2.43E-7
6 SGTC_786 4544-0069 4.97 423.0 µM 2.22E-7 11 3.41E-7
7 SGTC_978 1416-0512 4.83 87.4 µM 4.45E-7 redox potentiating 17 6.70E-7
8 SGTC_2434 5670830 4.69 169.9 µM 9.20E-7 12 1.35E-6
9 SGTC_2768 sulfasalazine 4.51 50.2 µM 2.26E-6 8 3.23E-6
10 SGTC_2942 9052777 4.31 62.2 µM 5.94E-6 28 8.21E-6
11 SGTC_2775 cyproheptadine 4.18 69.6 µM 1.07E-5 14 1.45E-5
12 SGTC_3211 9128781 4.08 49.5 µM 1.69E-5 53 2.26E-5
13 SGTC_882 0843-0131 4.04 351.0 µM 2.03E-5 16 2.69E-5
14 SGTC_864 0469-0573 3.98 172.0 µM 2.61E-5 20 3.44E-5
15 SGTC_2432 5630864 3.82 12.2 µM 5.14E-5 23 6.62E-5
16 SGTC_1677 st019204 3.72 53.9 µM 7.96E-5 26 1.01E-4
17 SGTC_2481 5478948 3.70 45.2 µM 8.66E-5 PDR1 43 1.10E-4
18 SGTC_66 0109-0187 3.64 42.4 µM 1.06E-4 47 1.34E-4
19 SGTC_1192 1488-0095 3.63 21.4 µM 1.11E-4 24 1.40E-4
20 SGTC_3272 9137413 3.51 49.5 µM 1.81E-4 24 2.24E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.253 2.49E-50 YOL100W PKH2 hom Serine/threonine protein kinase involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; redundant with Pkh1p
0.220 4.84E-38 YDR516C EMI2 hom Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.204 5.38E-33 YGL251C HFM1 hom Meiosis specific DNA helicase involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity
0.195 5.51E-30 YBR162C TOS1 hom Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C
0.194 1.05E-29 YGL250W RMR1 hom Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus
0.185 3.15E-27 YIL039W TED1 hom Conserved phosphoesterase domain-containing protein that acts together with Emp24p/Erv25p in cargo exit from the ER; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
0.165 5.89E-22 YGL236C MTO1 hom Mitochondrial protein, forms a heterodimer complex with Mss1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants
0.144 5.44E-17 YOR085W OST3 hom Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.139 5.59E-16 YNL067W RPL9B hom Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6
0.138 1.26E-15 YGR159C NSR1 hom Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis
0.115 2.36E-11 YKL096C-B_p YKL096C-B_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.115 2.87E-11 YDR281C PHM6 hom Protein of unknown function, expression is regulated by phosphate levels
0.113 6.38E-11 YDR443C SSN2 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation
0.110 1.52E-10 YLL045C RPL8B hom Ribosomal 60S subunit protein L8B; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication
0.110 1.55E-10 YGR206W MVB12 hom ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin