The major response signatures can be explored in detail in the network of response signatures (i.e. a web version of fig. 2).
Major response signatures and their associated screens are indicated below (click on arrow to view screens).
| Response Signature | Description | Number of Screens |
▶ |
60S ribosome export |
LSG1-NMD3 mediated ribosomal export from the nucleus |
102 |
|
|
▶ |
amide catabolism |
responses associated with DAL81, a nitrogen catabolite activator |
32 |
|
|
▶ |
anthracycline transcription coupled DNA repair |
responses associated with SSL2, a member of RNA polymerase transcription factor TFIIH holoenzyme, and DNA damage |
7 |
|
|
▶ |
azole & statin |
sterol biosynthesis, induced by ergosterol pathway inhibitors such as azoles and statins |
26 |
|
|
▶ |
calcium & mitochondrial duress |
calcium stress response, includes IMP2', a transcriptional activator involved in ion homeostasis |
38 |
|
|
▶ |
cell wall |
regulation of cell wall organization |
31 |
|
|
▶ |
cell wall signaling |
protein kinase C signaling |
25 |
|
|
▶ |
copper-dependent oxidative stress |
copper transport induced by oxidative stress and copper-dependent SOD1, SOD2 |
25 |
|
|
▶ |
DNA damage response |
DNA damage and repair |
34 |
|
|
▶ |
DNA intercalators |
responses associated with ERG12 (ergosterol biosynthesis), YMR166C (potential mitochondrial carrier), EFM1 (lysine methyltransferase of eIF1A) and CEP3 (kineticore protein) |
27 |
|
|
▶ |
endomembrane recycling |
responses associated with RCY1, a protein involved in recycling plasma membrane proteins, and (amino)phospholipid transport |
31 |
|
|
▶ |
ERAD & cell cycle |
endoplasmic reticulum-associated degradation and cell cycle |
11 |
|
|
▶ |
ERG2 |
responses associated with ERG2, involved in ergosterol biosynthesis; deletion increases membrane permeability; no other signature genes |
49 |
|
|
▶ |
ergosterol biosynthesis |
ergosterol biosynthesis |
20 |
|
|
▶ |
ergosterol depletion effects on membrane |
effects of high concentrations of ergosterol inhibitors on plasma membrane (e.g. vesicular trafficking, sterol biosynthesis) and regulation of GTPase activity |
15 |
|
|
▶ |
excess fatty acid |
response to excess fatty acid, primarily through LRO1, an acyltransferase that catalyzes diacylglycerol esterification |
9 |
|
|
▶ |
exosome |
exosome complex; induced by uracil analogs such as 5-fluorouracil |
7 |
|
|
▶ |
fatty acid desaturase (OLE1) |
responses associated with drug-induced happloinsufficiency of OLE1 (fatty acid desaturase) |
36 |
|
|
▶ |
Golgi |
intra-Golgi transport involving the COG1-8 Golgi transport complex |
71 |
|
|
▶ |
heat shock/prefoldin |
responses associated with the prefoldin complex, induced by heat shock |
5 |
|
Screen ID | Conditions | Concentration | Pubchem CID |
SGTC_238 | 5hr heat shock (37°C) + nsc-638432 | 8.93 µM | 368090 |
SGTC_239 | 5hr heat shock (37°C) + mitomycin C | 819.44 µM | 5746 |
SGTC_240 | 5hr heat shock (37°C) + Streptovitacin | 300 µM | 91467 |
SGTC_242 | 5hr heat shock (37°C) + 5-fluorouracil | 4.57 µM | 3385 |
SGTC_243 | 5hr heat shock (37°C) + splitomicin | 1 µM | 5269 |
|
▶ |
heme biosynthesis & mitochondrial translocase |
heme biosynthesis and the mitchondrial outer membrane translocase complex |
19 |
|
|
▶ |
heme requiring |
responses associated with heme biosynthesis, induced by compounds such as the iron chelator bathophenantroline |
14 |
|
|
▶ |
iron homeostasis |
responses associated with iron homeostasis, induced by several iron chelators |
35 |
|
|
▶ |
mitochondrial processes |
responses associated with mitochondrial processes including ATPase inhibition |
53 |
|
|
▶ |
mitochondrial response to ROS |
responses associated with mitochondrial genome maintenance, induced by compounds such as the antioxidant mangostin, which may participate in redox cycling |
16 |
|
|
▶ |
mitochondrial stress |
disruption of mitochondrial processes, associated with charged and reactive compounds |
10 |
|
|
▶ |
NEO1 |
notable drug-induced haploinsufficiency of NEO1, an aminophospholipid plasma membrane flippase |
21 |
|
|
▶ |
NEO1-PIK1 |
drug-induced haploinsufficiency of NEO1 (aminophospholipid plasma membrane flippase) and PIK1 (phosphatidylinositol 4-kinase) |
17 |
|
|
▶ |
PDR1 |
notable requirement for PDR1, a transcription factor that regulates the pleiotropic drug response; no other signature genes |
26 |
|
|
▶ |
plasma membrane duress |
piperazine-induced plasma membrane duress |
18 |
|
|
▶ |
redox potentiating |
protein catabolism via the multivesicular body sorting pathway associated with the generation of superoxide |
27 |
|
|
▶ |
RNA pol III & RNase P/MRP |
responses associated with RNA polymerase III and other complexes |
17 |
|
|
▶ |
RNA processing & uracil transport |
strong requirement for FUR4, a uracil transporter, and RNA processing genes GLC7 and RRP45; no other signature genes |
29 |
|
|
▶ |
RPP1 & pyrimidine depletion |
notable drug-induced haploinsufficiency of RPP1, a member of RNase P and MRP, complexes that cleave rRNA and tRNA precursors, respectively |
85 |
|
|
▼ |
RSC & ERG11 |
RSC chromatin structure remodeling complex and ERG11, an ergosterol biosynthesis enzyme |
16 |
|
|
▶ |
RSC complex & mRNA processing |
RSC chromatin structure remodeling complex and mRNA processing |
24 |
|
|
▶ |
sphingolipid biosynthesis & PDR1 |
responses associated with sphingolipid biosynthesis and PDR1, a transcription factor that regulates the pleiotropic drug response (induced by surfactants) |
14 |
|
|
▶ |
superoxide |
pentose phosphate pathway/NADP metabolism |
13 |
|
|
▶ |
SWF1 & branched chain AA biosynthesis |
branched chain amino acid biosynthesis and protein modification via SWF1 |
9 |
|
|
▶ |
translation |
cytoplasmic and mitochondrial translation |
6 |
|
|
▶ |
TRP & mitochondrial translation |
thiazolidinone targeting of protein prenylation (RER2) dirupts mitochondrial function |
14 |
|
|
▶ |
TSC3-RPN4 |
responses associated with a strong requirement for TSC3 (activator of sphingolipid biosynthesis) and a moderate requirement for RPN4 (transcription factor that stimulates expression of proteasome genes) |
59 |
|
|
▶ |
tubulin folding & SWR complex |
tubulin biogenesis, induced by tubulin inhibitors benomyl and nocodazole |
26 |
|
|
▶ |
ubiquinone biosynthesis & proteasome |
responses associated with the 20S proteosome, ubiquinone biosynthesis and an adamantyl structure moiety |
18 |
|
|
▶ |
unfolded protein response |
unfolded protein response induced by inhibition of diverse HIP targets |
11 |
|
|