Databases and Datasets

FAQs

Deletion Strains Available

References

Protocols &Technical Information

Useful Sites & Links

Consortium Members' Addresses

Yeast Deletion Database (consortium members only)

Original kanB and kanC primers

kanB

The kanB primer is homologous to the non-coding strand and is located 250 bp downstream of the 5 '-end of thekanMX4 module.

The "A-kanB" primer pair is used to check the upstream recombination junction.

kanB = 5 ' CTG CAG CGA GGA GCC GTA AT 3 '

kanC

The kanC primer is homologous to the coding strand and is located 587bp upstream of the 3 ' end of the kanMX4 module.

kanC = 5 ' - TGA TTT TGA TGA CGA GCG TAA T - 3'

Alternate KanB and KanC primers

kanB1 is at position 252 on the KanMX map

kanB1 = 5'- TGT ACG GGC GAC AGT CAC AT - 3'

For the size of the A-KanB1 product, subtract90 from the size of A-KanB given in the database

kanC3 is at position 1442 on the KanMX map

kanC3 = 5'- CCT CGA CAT CAT CTG CCC AGA T -3'

For the size of the KanC3-D product, subtract 450 from the size of KanC-D given in the database.

To order from Operon Technologies, click here.