Protocols &Technical Information
Yeast Deletion Database (consortium members only)
Screening the deletion mutants for
altered cell morphology
Deletion strains were grown individually at 30°C with 0.8ml liquid YPAD in 96-well microtiter plates with shaking (Dot Scientific, U.S.A.); each well contained one 3.5 mm glass bead to facilitate mixing. The cells were grown to stationary phase, then diluted and grown to mid-log-phase (at least six generations). Cells were fixed by the addition of formaldehyde to a final concentration of 3.7%, incubated for one hour at 30°C, washed with PBS, resuspended in PBS, and examined by phase-contrast and differential interference contrast microscopy.
last updated Jan03